Journal
NANOMATERIALS
Volume 12, Issue 4, Pages -Publisher
MDPI
DOI: 10.3390/nano12040648
Keywords
stiffness patterning; gray-leveled lithography; traction force microscopy; hydrogel; cell ADHESION; mechanosensitivity
Categories
Funding
- Agence Nationale de la Recherche [ANR-12-JSVE5-0008]
- CEA TechnoSante program
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Cell rigidity sensing is a basic cellular process that allows cells to adapt to mechanical cues by adjusting the level of stress. Researchers have developed an efficient method to design micron-scale stiffness patterns and a surface coating protocol to tune the density of adhesion proteins, providing tools for cells to exert force on their surroundings. These findings enhance our understanding of the contribution of stiffness alterations in diseases.
Cell rigidity sensing-a basic cellular process allowing cells to adapt to mechanical cuesinvolves cell capabilities exerting force on the extracellular environment. In vivo, cells are exposed to multi-scaled heterogeneities in the mechanical properties of the surroundings. Here, we investigate whether cells are able to sense micron-scaled stiffness textures by measuring the forces they transmit to the extracellular matrix. To this end, we propose an efficient photochemistry of polyacrylamide hydrogels to design micron-scale stiffness patterns with kPa/mu m gradients. Additionally, we propose an original protocol for the surface coating of adhesion proteins, which allows tuning the surface density from fully coupled to fully independent of the stiffness pattern. This evidences that cells pull on their surroundings by adjusting the level of stress to the micron-scaled stiffness. This conclusion was achieved through improvements in the traction force microscopy technique, e.g., adapting to substrates with a non-uniform stiffness and achieving a submicron resolution thanks to the implementation of a pyramidal optical flow algorithm. These developments provide tools for enhancing the current understanding of the contribution of stiffness alterations in many pathologies, including cancer.
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