Journal
NANOMATERIALS
Volume 11, Issue 11, Pages -Publisher
MDPI
DOI: 10.3390/nano11112888
Keywords
iron oxide nanoparticles; colloidal properties; cellular uptake; magnetic resonance imaging
Categories
Funding
- Fondo de Investigaciones Sanitarias [FIS [PI17/01489]]
- Miguel Servet Program [CP11/00147, CPII16/00056]
- Instituto de Salud Carlos III [RTC-2015-3846-1, RTC-2016-4990-1]
- Spanish Ministerio de Economia y Competitividad (MINECO)/FEDER funds [PID2019-106301RB-I00]
- Spanish Ministry of Science, Innovation and Universities [P2018/NMT-4321]
- Madrid Regional Government
- MINECO (FPI program fellowship) from Spain
- Ministerio de Ciencia e Innovacion, Spain [PID2019-110895RB-I00]
- Junta de Comunidades de Castilla-La Mancha, Spain [SBPLY/19/180501/000211]
- Donnell Foundation 21st Century Science Initiative in Mathematical and Complex Systems Approaches for Brain Cancer (USA) [220020560]
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Research on IONPs with different coatings and core sizes has provided insights for their application as negative contrast agents in clinical settings. The study found that specific core size and coating combinations yield the best results for MRI contrast enhancement.
Iron oxide nanoparticles (IONPs) are suitable materials for contrast enhancement in magnetic resonance imaging (MRI). Their potential clinical applications range from diagnosis to therapy and follow-up treatments. However, a deeper understanding of the interaction between IONPs, culture media and cells is necessary for expanding the application of this technology to different types of cancer therapies. To achieve new insights of these interactions, a set of IONPs were prepared with the same inorganic core and five distinct coatings, to study their aggregation and interactions in different physiological media, as well as their cell labelling efficiency. Then, a second set of IONPs, with six different core sizes and the same coating, were used to study how the core size affects cell labelling and MRI in vitro. Here, IONPs suspended in biological media experience a partial removal of the coating and adhesion of molecules. The FBS concentration alters the labelling of all types of IONPs and hydrodynamic sizes & GE; 300 nm provide the greatest labelling using the centrifugation-mediated internalization (CMI). The best contrast for MRI results requires a core size range between 12-14 nm coated with dimercaptosuccinic acid (DMSA) producing R-2* values of 393.7 s(-1) and 428.3 s(-1), respectively. These findings will help to bring IONPs as negative contrast agents into clinical settings.
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