4.7 Article

Tetrahedral DNA Framework-Programmed Electrochemical Biosenors with Gold Nanoparticles for Ultrasensitive Cell-Free DNA Detection

Journal

NANOMATERIALS
Volume 12, Issue 4, Pages -

Publisher

MDPI
DOI: 10.3390/nano12040666

Keywords

tetrahedral DNA framework; gold nanoparticles; hybridization chain reaction; cell-free DNA; electrochemical biosenors

Funding

  1. Strategic Priority Research Program of the Chinese Academy of Sciences
  2. Program of Shanghai Academic/Technology Research Leader [20XD1404600]
  3. Shanghai Municipal Science and Technology Commission [20511107600, 19511107100, 19511107102]
  4. Chinese Academy of Science [KFJ-STS-QYZD-2021-08-002]
  5. National Key Research and Development Program of China [2016YFC0100600]
  6. Hundred-Talent Program (Shanghai University of Medicine & Health Sciences) [ZPBRK-18-04]

Ask authors/readers for more resources

This study developed an ultrasensitive cfDNA electrochemical biosensor based on TDF-modified gold nanoparticles for detecting cfDNA related to breast cancer. By controlling the number of base pairs on DNA frameworks and combining with HCR for signal amplification, the sensor achieved an ultra-low detection limit and excellent discrimination ability. It also showed potential for clinical research by detecting cfDNA in mimic serum samples.
Tumor-associated cell-free DNA (cfDNA) is a dynamic biomarker for genetic analysis, early diagnosis and clinical treatment of cancers. However, its detection has limitations because of its low abundance in blood or other complex bodily fluids. Herein, we developed an ultrasensitive cfDNA electrochemical biosensor (E-cfDNA sensor) based on tetrahedral DNA framework (TDF)-modified gold nanoparticles (Au NPs) with an interface for cfDNA detection. By accurately controlling the numbers of base pairs on each DNA framework, three types of TDFs were programmed: 26 base pairs of TDF; 17 base pairs of TDF; and 7 base pairs of TDF (TDF-26, TDF-16 and TDF-7, respectively). We also combined the TDF with hybridization chain reaction (HCR) to achieve signal amplification. Under optimal conditions, we detected the breast cancer susceptibility gene 1 (BRCA-1), a representative cfDNA closely related to breast cancer. An ultra-low detection limit of 1 aM with a linear range from 1 aM to 1 pM by TDF-26 was obtained, which was superior to the existing methods. Each type of TDF has excellent discrimination ability, which can distinguish single mismatch. More significantly, we also detected BRCA-1 in mimic serum samples, demonstrating that the E-cfDNA sensor has potential use in clinical research.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available