Development of Silica Nanoparticle Supported Imprinted Polymers for Selective Lysozyme Recognition

Protein imprinted MIPs show notable potential for applications in many analytical areas such as clinical analysis, medical diagnostics and environmental monitoring, but also in drug delivery scenarios. In this study, we present various modifications of two different synthesis routes to create imprinted core-shell particles serving as a synthetic recognition material for the protein hen egg white (HEW) lysozyme. HEW lysozyme is used as food additive E 1105 for preservation due to its antibacterial effects. For facilitating quality and regulatory control analysis in food matrices, it is necessary to apply suitable isolation methods as potentially provided by molecularly imprinted materials. The highest binding capacity achieved herein was 58.82 mg/g with imprinting factors ranging up to 2.74, rendering these materials exceptionally suitable for selectively isolating HEW lysozyme.

Automated summary

Protein imprinted MIPs have potential applications in clinical analysis, medical diagnostics, environmental monitoring, and drug delivery. The study focuses on creating imprinted core-shell particles for selectively isolating HEW lysozyme, which is used as a food additive for its antibacterial effects. The highest binding capacity achieved was 58.82 mg/g, making these materials suitable for quality control analysis in food matrices.