Journal
GENES
Volume 13, Issue 2, Pages -Publisher
MDPI
DOI: 10.3390/genes13020324
Keywords
ovum pick-up; cathepsin B; E-64; in vitro maturation; developmental competence; bovine oocytes
Categories
Funding
- JSPS (KAKENHI) [24580439]
- Cooperative Research Program for Agriculture Science & Technology Development [RDA PJ01029305]
- JSPS fund, Japan [P15400]
Ask authors/readers for more resources
Supplementation of E-64 significantly improves the developmental competence of OPU-derived immature oocytes and enhances the quality of blastocysts, suggesting its potential as a tool to enhance the efficiency of OPU-IVF program.
Recovery of bovine oocytes using the ovum pick-up (OPU) technique offers the advantage of rapid genetic improvement through propagation of desired genes from animals with high genetic qualities. However, the developmental competence of OPU-derived immature oocytes remains relatively poor. We previously found that cathepsin B gene expression and activity are increased in poor quality oocytes and embryos compared to good quality ones. In this study, we investigated the effect of E-64 (cathepsin B inhibitor) supplementation during in vitro maturation (IVM) on the developmental competence of OPU-derived immature oocytes and the quality of the produced blastocysts. Our results showed that supplementation of IVM medium with E-64 significantly improved the developmental competence of OPU-derived immature oocytes as evidenced by the significant increase of the blastocyst rate. Importantly, the presence of E-64 during IVM also significantly improved blastocyst quality by increasing the total cell number and decreasing the percentage of TUNEL positive cells. These results indicate that E-64 supplementation during IVM is a promising tool to improve the efficiency of OPU-IVF program by improving the developmental competence of OPU-derived immature oocytes.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available