Journal
GENES
Volume 13, Issue 1, Pages -Publisher
MDPI
DOI: 10.3390/genes13010139
Keywords
Arabidopsis; chloroplast; ndhB-C149; OTP970; PPR; RNA editing
Categories
Funding
- National Natural Science Foundation of China [31970261, 31770260]
- Talent Support Project of Guangdong [2019TQ05N182]
Ask authors/readers for more resources
RNA editing is essential for compensating for defects or mutations in haploid organelle genomes. In this study, OTP970, a plastid-targeted PPR protein, was identified as a key player in RNA editing in plastids. The DYW domain of OTP970 was found to be essential for its function.
RNA editing is essential for compensating for defects or mutations in haploid organelle genomes and is regulated by numerous trans-factors. Pentatricopeptide repeat (PPR) proteins are the prime factors that are involved in RNA editing; however, many have not yet been identified. Here, we screened the plastid-targeted PLS-DYW subfamily of PPR proteins belonging to Arabidopsis thaliana and identified ORGANELLE TRANSCRIPT PROCESSING 970 (OTP970) as a key player in RNA editing in plastids. A loss-of-function otp970 mutant was impaired in RNA editing of ndhB transcripts at site 149 (ndhB-C149). RNA-immunoprecipitation analysis indicated that OTP970 was associated with the ndhB-C149 site. The complementation of the otp970 mutant with OTP970 lacking the DYW domain (OTP970(Delta DYW)) failed to restore the RNA editing of ndhB-C149. ndhB gene encodes the B subunit of the NADH dehydrogenase-like (NDH) complex; however, neither NDH activity and stability nor NDH-PSI supercomplex formation were affected in otp970 mutant compared to the wild type, indicating that alteration in amino acid sequence is not necessary for NdhB function. Together, these results suggest that OTP970 is involved in the RNA editing of ndhB-C149 and that the DYW domain is essential for its function.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available