4.3 Article

Dynamic shapes of the zygote and two-cell mouse and human

Journal

BIOLOGY OPEN
Volume 10, Issue 12, Pages -

Publisher

COMPANY BIOLOGISTS LTD
DOI: 10.1242/bio.059013

Keywords

Mouse zygote; Morphokinetics; Shape cycles; Pronuclear fading; Human; Cytoplasm vortices

Categories

Funding

  1. Polish Academy Science Centre [UMO-2017/27/B/NZ5/00405]

Ask authors/readers for more resources

Morphokinetics of mouse zygotes were studied at different stages, revealing changing patterns of shape and area, as well as the development of vortices during cell division. These shape and area cycles may play a role in promoting exchange with the environment, while the persistent circulation in the cytoplasm could aid in communication between the cell's interior and periphery.
Mouse zygote morphokinetics were measured during interphase, the mitotic period, cytokinesis, and two-cell stage. Sequences of rounder-distorted-rounder shapes were revealed, as were changing patterns of cross section area. A calcium chelator and an actin-disrupting agent inhibited the area changes that occurred between pronuclear envelope breakdown and cytokinesis. During cell division, two vortices developed in each nascent cell and they rotated in opposite directions at each end of the cell, a pattern that sometimes persisted for up to 10 h. Exchange with the environment may have been promoted by these shape and area cycles and persisting circulation in the cytoplasm may have a similar function between a cell's interior and periphery. Some of these movements were sporadically also seen in human zygotes with abnormal numbers of pronuclei and the two-cell stages that developed from these compromised human zygotes.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.3
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available