Journal
ADVANCED MATERIALS INTERFACES
Volume 8, Issue 21, Pages -Publisher
WILEY
DOI: 10.1002/admi.202101191
Keywords
calcium carbonate; charge-based cell capture; circulating tumor cells; magnetic nanoparticles; pH-sensitive release
Funding
- National Natural Science Foundation of China [32071395]
- Fundamental Research Funds for the Central Universities - Outstanding Clinical Discipline Project of Shanghai Pudong [PWYgy2018-02]
- Science and Technology Commission of Shanghai Municipality [19DZ1910502]
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A novel nanoplatform has been developed for isolation and pH-sensitive release of CTCs, eliminating the need for extra acidic substances. The nanoplatform showed efficient release of CTCs without compromising bioefficacy, performing well in both mimic blood samples and clinical samples from cancer patients.
Circulating tumor cells (CTCs) have been recognized as significant research target for cancer prognosis and metastasis mechanism. Efficient and all-subpopulations-covered isolation and facile release of CTCs with good viability are pivotal for profound mechanism research and molecular profiling. However, integrated nanoplatform with these two functions is yet to be elucidated. Among existing release techniques including physical, biological, and chemical methods, acid responsive release of CTCs attracts intensive interests due to its straightforward and easily accessible feature. However, extra acidic substance seems indispensable so far, which poses potential hazard to the rare and susceptible CTCs. To address this challenge, polyelectrolyte functionalized CaCO3 coated magnetic nanocrystal clusters are developed as the nanoplatform for multi-subpopulations isolation and pH-sensitive release of CTCs. Only buffer solution components are needed rather than other acidic substance to provide mild acidic environment with pH value of 6.5, facilitate highly efficient release. Moreover, the bioefficacy of CTC capture and mild condition recovery is not compromised by formation of protein corona on the cell-friendly nanoplatform. This nanoplatform exhibits good performance in both mimic blood samples and cancer patients' clinical samples.
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