Journal
ADVANCED MATERIALS INTERFACES
Volume 9, Issue 18, Pages -Publisher
WILEY
DOI: 10.1002/admi.202101677
Keywords
F-19-MRI; coatings; fluorinated nanoparticles; fluorine; hydrophobin; protein corona; self-assembly
Funding
- Regione Lombardia POR FESR [1175999]
- MIUR [2017MYBTXC]
- Politecnico di Milano within the CRUI-CARE Agreement
- Italian Ministry of Health [GR-2016-02361325]
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Fluorine-magnetic resonance imaging (F-19-MRI) is a promising diagnostic technique that can be easily translated for clinical use, requiring appropriate delivery systems for highly omniphobic fluorinated probes. The use of the film-forming protein hydrophobin offers a sustainable method to change the wettability of fluorinated surfaces, demonstrated here to stabilize F-19-MRI probes in aqueous solution as nanoparticles for imaging applications.
In recent years, fluorine-magnetic resonance imaging (F-19-MRI) has emerged as a promising diagnostic technique, complementary to traditional proton magnetic resonance imaging (H-1-MRI) and easily translatable for clinical use, providing in-depth in vivo quantification without the use of radioactive agents. This creates a need for the development of appropriate delivery systems for highly omniphobic fluorinated probes. The use of the film-forming protein hydrophobin (HFBII) represents a sustainable and simple method to invert the philicity of fluorinated surfaces. Here, the ability of HFBII to form a rigid protein monolayer on superfluorinated coatings rendering them hydrophilic is shown, a property that is also retained in biological environment. This approach is then translated to directly disperse a solid superfluorinated F-19-MRI probe, PERFECTA, in aqueous solution through the formation of core-shell hydrophobin stabilized PERFECTA nanoparticles (NPs). The obtained NPs are fully characterized in terms of morphology, magnetic properties, colloidal stability, protein corona formation, cellular viability, and imaging performance.
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