Journal
STEM CELLS INTERNATIONAL
Volume 2021, Issue -, Pages -Publisher
HINDAWI LTD
DOI: 10.1155/2021/9941733
Keywords
-
Categories
Funding
- Shenzhen Key Medical Subject [SZXK023]
- Shenzhen San-Ming Project of Medicine [SZSM201612092]
- National Natural Science Foundation of China [82001319]
- Guangdong Basic and Applied Basic Research Foundation [2019A1515011290, 2019A1515110983]
- Bethune Charitable Foundation
- CSPC Osteoporosis Research Foundation Project [G-X-2020-1107-21]
- Shenzhen Scientific Research Project of Health and Family Planning System [SZXJ2018077]
Ask authors/readers for more resources
The study found that SOX12 is increased in osteosarcoma, with high expression levels associated with poor prognosis and high recurrence rates. SOX12 promotes stem cell-like phenotypes and osteosarcoma tumor growth by upregulating JAGGED1.
SOX12 plays a role in promoting the growth of some tumors; however, its role in osteosarcoma remains unclear. From gene expression omnibus (GEO) and tumor alterations relevant for genomics-driven therapy (TARGET) databases, Kaplan-Meier analyses were conducted to establish relationships between SOX12 expression and osteosarcoma survival and recurrence in osteosarcoma patients. We also performed in vitro and in vivo assays to determine SOX12 function in osteosarcoma etiology. SOX12 expression was increased in osteosarcoma; high SOX12 expression levels were related to a poor prognosis and a high disease recurrence in patients. Moreover, SOX12 expression in osteosarcoma cell lines was increased, similar to osteosarcoma cancer stem cells. We also observed that SOX12 knockdown inhibited the spheroidization and expression of stemness markers in osteosarcoma cells and tumor formation in nude mice. In addition, SOX12 knockdown inhibited JAGGED1 and HES1 expression. Similarly, JAGGED1 knockdown also inhibited the formation of osteosarcoma cancer stem cells into pellets and reduced the expression of stemness markers and tumor formation capabilities in nude mice. Finally, during SOX12 knockdown, JAGGED1 overexpression rescued osteosarcoma cells from spheroidizing. SOX12 promotes stem cell-like phenotypes and osteosarcoma tumor growth by upregulating JAGGED1.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available