4.7 Article

The impact of anionic polymers on gene delivery: how composition and assembly help evading the toxicity-efficiency dilemma

Journal

JOURNAL OF NANOBIOTECHNOLOGY
Volume 19, Issue 1, Pages -

Publisher

BMC
DOI: 10.1186/s12951-021-00994-2

Keywords

Gene delivery; Cationic polymer; Micelle; Transfection; Anionic polymer; Shielding

Funding

  1. Bundesministerium fur Bildung und Forschung (BMBF, Germany) [13XP5034A PolyBioMik]
  2. German Research Foundation (DFG, Emmy-Noether Programme) [358263073]
  3. DFG [316213987]
  4. Deutsche Forschungsgemeinschaft (DFG)
  5. European Fund for Regional Development (EFRE)
  6. DFG
  7. Thuringer Aufbaubank (TAB)
  8. Projekt DEAL

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Cationic polymers have been extensively studied for non-viral gene delivery, but their charged nature can lead to increased cytotoxicity and limited in vivo application. By utilizing hydrophilic or anionic shielding polymers, the toxicity of cationic polymers can be reduced, improving gene transfection efficiency. The study demonstrated the potential of using layering micelles with shielding polymers for gene delivery applications.
Cationic polymers have been widely studied for non-viral gene delivery due to their ability to bind genetic material and to interact with cellular membranes. However, their charged nature carries the risk of increased cytotoxicity and interaction with serum proteins, limiting their potential in vivo application. Therefore, hydrophilic or anionic shielding polymers are applied to counteract these effects. Herein, a series of micelle-forming and micelle-shielding polymers were synthesized via RAFT polymerization. The copolymer poly[(n-butyl acrylate)-b-(2-(dimethyl amino)ethyl acrylamide)] (P(nBA-b-DMAEAm)) was assembled into cationic micelles and different shielding polymers were applied, i.e., poly(acrylic acid) (PAA), poly(4-acryloyl morpholine) (PNAM) or P(NAM-b-AA) block copolymer. These systems were compared to a triblock terpolymer micelle comprising PAA as the middle block. The assemblies were investigated regarding their morphology, interaction with pDNA, cytotoxicity, transfection efficiency, polyplex uptake and endosomal escape. The naked cationic micelle exhibited superior transfection efficiency, but increased cytotoxicity. The addition of shielding polymers led to reduced toxicity. In particular, the triblock terpolymer micelle convinced with high cell viability and no significant loss in efficiency. The highest shielding effect was achieved by layering micelles with P(NAM-b-AA) supporting the colloidal stability at neutral zeta potential and completely restoring cell viability while maintaining moderate transfection efficiencies. The high potential of this micelle-layer-combination for gene delivery was illustrated for the first time.

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