Journal
JOURNAL OF CHEMISTRY
Volume 2022, Issue -, Pages -Publisher
HINDAWI LTD
DOI: 10.1155/2022/2673396
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Methylation at the C5 position of cytosine (5 mC) is a common epigenetic modification process linked to many human diseases. A recent discovery shows that DNA methyltransferases (DNMTs) can add a methyl group at position 3 to yield 3 mC, which can cause severe damages to DNA. By using density functional theory (DFT) modeling studies, we have gained insight into the mechanism of this new methylation approach and discovered an important off-target activity of DNMTs.
Methylation at C5 position of cytosine (5 mC) is the most abundantly occurring methylation process at CpG island, which has been well known as an epigenetic modification linked to many human diseases. Recently, another methylation approach has been discovered to show that DNA methyltransferases (DNMTs) promote the addition of the methyl group at position 3 to yield 3 mC. The existence of 3 mC can cause severe damages to the DNA strand, such as blocking its replication, repair, and transcription, affecting its stability, and initiating a double-strand DNA break. To gain a deeper insight into the formation of 3 mC, we have performed density functional theory (DFT) modeling studies at different levels of theory to clearly map out the mechanistic details for this new methylation approach. Our computed results are in harmony with pertinent experimental observations and shed light on a crucial off-target activity of DNMTs.
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