Journal
INTERNATIONAL JOURNAL OF GENOMICS
Volume 2022, Issue -, Pages -Publisher
HINDAWI LTD
DOI: 10.1155/2022/4565977
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Funding
- Adaptable and Seamless Technology Transfer Program (A-STEP), Industry-Academia Joint Promotion Stage, High Risk Challenge grant by the Japan Science and Technology Agency (JST) [14529973]
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We identified the key genes controlling the late maturation of the Japonica cultivar Isehikari and elucidated their effect on maturity. This study provides important genetic information for a better understanding and improvement of rice cultivation.
We identified the key genes controlling the late maturation of the Japonica cultivar Isehikari, which was found at Ise Jingu Shrine and matures 6 days later than Koshihikari. We conducted a genetics-based approach through this study. First, the latest mature plants, which flowered later than Isehikari, were segregated in the F-2 and F-3 generations of KoshihikarixIsehikari. Next, the linkage relationship of a single late-maturing gene with the SSR markers on the long arm of chromosome 3 was inferred by using late-maturing homozygous F-2 segregants. Moreover, genetic analyses of late maturity were conducted through the process of six times of continuous backcross with Koshihikari as a recurrent parent by using the late-maturing homozygous F-3 line as a nonrecurrent parent, thus developing a late-maturing isogenic Koshihikari (BC6F2). As a result, we elucidated a single late-maturing gene with incomplete dominance that caused the 14-day maturation delay of Koshihikari. The whole-genome sequencing was conducted on both of Koshihikari and the late-maturing isogenic Koshihikari. Then, the SNP call was conducted as the reference genome of Koshihikari. Finally, a single SNP was identified in the key gene Hd16 of the late-maturing isogenic Koshihikari.
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