Journal
FRONTIERS IN PLANT SCIENCE
Volume 12, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fpls.2021.791390
Keywords
abiotic stress; drought stress; Betula platyphylla; transcription factor; BpMYB123
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Funding
- China National Key RD Programme [2021YFD2200006]
- Heilongjiang Touyan Innovation Team Program (Tree Genetics and Breeding Innovation Team)
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The study identified a R2R3 MYB transcription factor gene, BpMYB123, from Betula platyphylla and revealed its significant role in drought stress. Overexpression of BpMYB123 enhances tolerance to drought stress by regulating BpLEA14, leading to increased peroxidase and superoxide dismatase activities and decreased oxidative damage in birch plants.
Drought stress causes various negative impacts on plant growth and crop production. R2R3-MYB transcription factors (TFs) play crucial roles in the response to abiotic stress. However, their functions in Betula platyphylla haven't been fully investigated. In this study, a R2R3 MYB transcription factor gene, BpMYB123, was identified from Betula platyphylla and reveals its significant role in drought stress. Overexpression of BpMYB123 enhances tolerance to drought stress in contrast to repression of BpMYB123 by RNA interference (RNAi) in transgenic experiment. The overexpression lines increased peroxidase (POD) and superoxide dismatase (SOD) activities, while decreased hydrogen peroxide (H2O2), superoxide radicals (O-2(-)), electrolyte leakage (EL) and malondialdehyde (MDA) contents. Our study showed that overexpression of BpMYB123 increased BpLEA14 gene expression up to 20-fold due to BpMYB123 directly binding to the MYB1AT element of BpLEA14 promoter. These results indicate that BpMYB123 acts as a regulator via regulating BpLEA14 to improve drought tolerance in birch.
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