Mung Bean (Vigna radiata L.) Source Leaf Adaptation to Shading Stress Affects Not Only Photosynthetic Physiology Metabolism but Also Control of Key Gene Expression

Shading stress strongly limits the effective growth of plants. Understanding how plant morphogenesis and physiological adaptation are generated in response to the reduced low light conditions is important for food crop development. In this study, two mung bean (Vigna radiata L.) cultivars, namely, Xilv 1 and Yulv 1, were grown in the field to explore the effects of shading stress on their growth. The results of morphology, physiology, and biochemistry analyses showed that the shading stress significantly weakened the leaf photosynthetic capacity as measured by the decreased net photosynthetic rate, stomatal conductance, and transpiration rate and increased intercellular CO2 concentration. These responses resulted in plant morphological characteristics that increased the light energy absorption in low light conditions. Such variations occurred due to the leaf anatomical structure with destroyed palisade tissues and spongy tissues. Under shading stress, Yulv 1 showed higher physiological metabolic intensity than Xilv 1, which was related to changes in chlorophyll (Chl), such as Chl a and b, and Chl a/b ratio. Compared with normal light conditions, the Chl fluorescence values, photosynthetic assimilation substances, and enzyme activities in mung bean plants under shading stress were reduced to different extent. In addition, the relative expression levels of VrGA2ox, VrGA20ox1, VrGA3ox1, VrROT3, and VrBZR1, which are related to endogenous hormone in mung bean leaves, were upregulated by shading stress, further leading to the improvements in the concentrations of auxin, gibberellins (GAs), and brassinolide (BR). Combined with the morphological, physiological, and molecular responses, Yulv 1 has stronger tolerance and ecological adaptability to shading stress than Xilv 1. Therefore, our study provides insights into the agronomic traits and gene expressions of mung bean cultivars to enhance their adaptability to the shading stress.

Automated summary

This study explored the effects of shading stress on the growth of two mung bean cultivars. The results showed that shading stress significantly weakened the leaf photosynthetic capacity and led to morphological and physiological changes in the plants. Yulv 1 exhibited stronger tolerance and ecological adaptability to shading stress compared to Xilv 1, which was related to changes in chlorophyll content and hormone concentrations.