Physiological, Biochemical, and Root Proteome Networks Revealed New Insights Into Salt Tolerance Mechanisms in Pongamia pinnata (L.) Pierre

Cultivation of potential biofuel tree species such as Pongamia pinnata would rehabilitate saline marginal lands toward economic gains. We carried out a physiological, biochemical, and proteomic analysis to identify key regulatory responses which are associated with salt tolerance mechanisms at the shoot and root levels. Pongamia seedlings were grown at 300 and 500 mM NaCl (similar to 3% NaCl; sea saline equivalent) concentrations for 15 and 30 days, gas exchange measurements including leaf net photosynthetic rate (A(sat)), stomatal conductance (g(s)), and transpiration rate (E), and varying chlorophyll a fluorescence kinetics were recorded. The whole root proteome was quantified using the free-labeled nanoLC-MS/MS technique to investigate crucial proteins involved in signaling pathways associated with salt tolerance. Pongamia showed no visible salt-induced morphological symptoms. However, Pongamia showed about 50% decline in gas exchange parameters including A(sat), E, and g(s) 15 and 30 days after salt treatment (DAS). The maximum potential quantum efficiency of photosystem (PS) II (Fv/Fm) was maintained at approximately 0.8 in salt-treated plants. The thermal component of PSII (DIo) was increased by 1.6-fold in the salt-treated plants. A total of 1,062 protein species were identified with 130 commonly abundant protein species. Our results also elucidate high abundance of protein species related to flavonoid biosynthesis, seed storage protein species, and carbohydrate metabolism under salt stress. Overall, these analyses suggest that Pongamia exhibited sustained leaf morphology by lowering net photosynthetic rates and emitting most of its light energy as heat. Our root proteomic results indicated that these protein species were most likely recruited from secondary and anaerobic metabolism, which could provide defense for roots against Na+ toxicity under salt stress conditions.

Automated summary

The study found that the potential biofuel tree species Pongamia pinnata displayed salt tolerance characteristics in a high salinity environment, with significant declines in gas exchange parameters but sustained leaf morphology and elevated heat emission. Proteomic analysis of roots suggested that Pongamia might defend against Na+ toxicity through proteins derived from secondary and anaerobic metabolism under salt stress conditions.