Oral Immunization With a Plant HSP90-SAG1 Fusion Protein Produced in Tobacco Elicits Strong Immune Responses and Reduces Cyst Number and Clinical Signs of Toxoplasmosis in Mice

Plant 90kDa heat shock protein (HSP90) is a potent adjuvant that increases both humoral and cellular immune responses to diverse proteins and peptides. In this study, we explored whether Arabidopsis thaliana HSP90 (AtHsp81.2) can improve the immune effects of a Toxoplasma gondii surface antigen 1 (SAG1). We designed two constructs containing the sequence of mature antigen (SAG1(m)), from aa(77) to aa(322,) and B- and T-cell antigenic epitope-containing SAG1(HC), from aa(221) to aa(319) fused to AtHsp81.2 sequence. When comparing the transient expression in Nicotiana tabacum X-27-8 leaves, which overexpress the suppressor helper component protease HC-Pro-tobacco etch virus (TEV), to that in N. benthamiana leaves, co-agroinfiltrated with the suppressor p19, optimal conditions included 6-week-old N. benthamiana plants, 7-day time to harvest, Agrobacterium tumefaciens cultures with an OD600nm of 0.6 for binary vectors and LED lights. While AtHsp81.2-SAG1(m) fusion protein was undetectable by Western blot in any of the evaluated conditions, AtHsp81.2-SAG1(HC) was expressed as intact fusion protein, yielding up to 90 mu g/g of fresh weight. Besides, the AtHsp81.2-SAG1(HC) mRNA was strongly expressed compared to the endogenous Nicotiana tabacum elongation factor-alpha (NtEF alpha) gene, whereas the AtHsp81.2-SAG1(m) mRNA was almost undetectable. Finally, mice were orally immunized with AtHsp81.2-SAG1(HC)-infiltrated fresh leaves (plAtHsp81.2-SAG1(HC) group), recombinant AtHsp81.2-SAG1(HC) purified from infiltrated leaves (rAtHsp81.2-SAG1(HC) group), non-infiltrated fresh leaves (control group), or phosphate-buffered saline (PBS group). Serum samples from plAtHsp81.2-SAG1(HC)-immunized mice had significantly higher levels of IgGt, IgG2a, and IgG2b anti-SAG1(HC) antibodies than serum from rAtHsp81.2-SAG1(HC), control, and PBS groups. The number of cysts per brain in the plAtHsp81.2-SAG1(HC)-immunized mice was significantly reduced, and the parasite load in brain tissue was also lower in this group compared with the remaining groups. In an immunoblot assay, plant-expressed AtHsp81.2-SAG1(HC) was shown to react with antibodies present in sera from T. gondii-infected people. Therefore, the plant expression of a T. gondii antigen fused to the non-pathogenic adjuvant and carrier plant HSP90 as formulations against T. gondii can improve the vaccine efficacy, and plant extract can be directly used for vaccination without the need to purify the protein, making this platform a suitable and powerful biotechnological system for immunogenic antigen expression against toxoplasmosis.

Automated summary

Plant-expressed HSP90 protein acts as a potent adjuvant to improve immune effects of Toxoplasma gondii antigen SAG1. Fusion protein AtHsp81.2-SAG1(HC) showed significant enhancement in antibody levels and reduced parasite load in mice compared to control groups. Plant-expressed AtHsp81.2-SAG1(HC) can directly be used as a potent biotechnological system for immunogenic antigen expression against toxoplasmosis.