Journal
FRONTIERS IN PLANT SCIENCE
Volume 12, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fpls.2021.796248
Keywords
R2R3-MYB gene family; Gynostemma pentaphyllum; gypenoside; flavonol; transcription factor
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Funding
- Natural Science Foundation of Guangxi Zhuang Autonomous Region [2020GXNSFBA297025]
- Guangxi Middle-aged and Young Teachers' Basic Ability Promotion Project [2020KY07039]
- Guangxi University of Chinese Medicine Scientific Research Fund [2019BS007]
- Special Fund for Introducing Scientific and Technological Talents of Guangdong Academy of Agricultural Sciences [R2020YJ-YB3003]
- Guangxi Key Laboratory of Zhuang and Yao Ethnic Medicine Open Project Fund [20-065-14]
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This study systematically analyzed the R2R3-MYB gene family in Gynostemma pentaphyllum and discovered that GpMYB81 plays a dual role in regulating gypenoside and flavonol biosynthesis.
The R2R3-MYB gene family participates in several plant physiological processes, especially the regulation of the biosynthesis of secondary metabolites. However, little is known about the functions of R2R3-MYB genes in Gynostemma pentaphyllum (G. pentaphyllum), a traditional Chinese medicinal herb that is an excellent source of gypenosides (a class of triterpenoid saponins) and flavonoids. In this study, a systematic genome-wide analysis of the R2R3-MYB gene family was performed using the recently sequenced G. pentaphyllum genome. In total, 87 R2R3-GpMYB genes were identified and subsequently divided into 32 subgroups based on phylogenetic analysis. The analysis was based on conserved exon-intron structures and motif compositions within the same subgroup. Collinearity analysis demonstrated that segmental duplication events were majorly responsible for the expansion of the R2R3-GpMYB gene family, and Ka/Ks analysis indicated that the majority of the duplicated R2R3-GpMYB genes underwent purifying selection. A combination of transcriptome analysis and quantitative reverse transcriptase-PCR (qRT-PCR) confirmed that Gynostemma pentaphyllum myeloblastosis 81 (GpMYB81) along with genes encoding gypenoside and flavonol biosynthetic enzymes exhibited similar expression patterns in different tissues and responses to methyl jasmonate (MeJA). Moreover, GpMYB81 could bind to the promoters of Gynostemma pentaphyllum farnesyl pyrophosphate synthase 1 (GpFPS1) and Gynostemma pentaphyllum chalcone synthase (GpCHS), the key structural genes of gypenoside and flavonol biosynthesis, respectively, and activate their expression. Altogether, this study highlights a novel transcriptional regulatory mechanism that suggests that GpMYB81 acts as a dual-function regulator of gypenoside and flavonol biosynthesis in G. pentaphyllum.
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