Journal
FRONTIERS IN PLANT SCIENCE
Volume 12, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fpls.2021.773553
Keywords
Senna occidentalis; anthraquinone; secondary metabolite; transcriptome analysis; transcription factor
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Funding
- National Institute of Agricultural Sciences [PJ015713]
- BioGreen21 Agri-Tech Innovation Program [PJ015710]
- Rural Development Administration, South Korea
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Through RNA-Seq and Iso-Seq analysis, a transcriptomic analysis of S. occidentalis was performed, identifying genes related to the anthraquinone biosynthesis pathway and transcription factor families. Tissue-specific differential expression analysis and measurement of anthraquinone levels revealed the relationship between gene expression and anthraquinone accumulation, as well as the differences in anthraquinones produced by S. occidentalis and S. tora.
Senna occidentalis is an annual leguminous herb that is rich in anthraquinones, which have various pharmacological activities. However, little is known about the genetics of S. occidentalis, particularly its anthraquinone biosynthesis pathway. To broaden our understanding of the key genes and regulatory mechanisms involved in the anthraquinone biosynthesis pathway, we used short RNA sequencing (RNA-Seq) and long-read isoform sequencing (Iso-Seq) to perform a spatial and temporal transcriptomic analysis of S. occidentalis. This generated 121,592 RNA-Seq unigenes and 38,440 Iso-Seq unigenes. Comprehensive functional annotation and classification of these datasets using public databases identified unigene sequences related to major secondary metabolite biosynthesis pathways and critical transcription factor families (bHLH, WRKY, MYB, and bZIP). A tissue-specific differential expression analysis of S. occidentalis and measurement of the amount of anthraquinones revealed that anthraquinone accumulation was related to the gene expression levels in the different tissues. In addition, the amounts and types of anthraquinones produced differ between S. occidentalis and S. tora. In conclusion, these results provide a broader understanding of the anthraquinone metabolic pathway in S. occidentalis.
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