4.6 Article

Biological Characterization of Yellow Fever Viruses Isolated From Non-human Primates in Brazil With Distinct Genomic Landscapes

Journal

FRONTIERS IN MICROBIOLOGY
Volume 13, Issue -, Pages -

Publisher

FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2022.757084

Keywords

yellow fever virus; Brazil outbreaks; genetic markers; virulence; mouse neurovirulence; cell infection; interferon-mediated viral inhibition

Categories

Funding

  1. Preventing and Combating the Zika Virus, MCTIC/FNDCT-CNPq/MEC524 CAPES/MS-Decit [440865/2016-6]
  2. Conselho Nacional de Desenvolvimento Cientifico e 525 Tecnologico [312446/2018]
  3. Preventing and Combating the Zika Virus MCTIC/FNDCT526 CNPq/MEC-CAPES/MSDecit [88881130684/2016-00]
  4. INOVA Geracao de Conhecimento, Fiocruz [VPPIS-004-FIO18]
  5. CNPq
  6. [MCTI/FINEP/FNDCT 01/2016 -ZIKA]
  7. [04.16.0058.00]

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This study found differences in infectivity and neurovirulence of yellow fever virus isolates from different regions in mammalian and mosquito cells, suggesting the presence of genomic markers that increase host infectivity in certain strains.
Since the beginning of the XXI Century, the yellow fever virus (YFV) has been cyclically spreading from the Amazon basin to Brazil's South and Southeast regions, culminating in an unprecedented outbreak that started in 2016. In this work, we studied four YFV isolated from non-human primates obtained during outbreaks in the states of Rio Grande do Sul in 2008 (PR4408), Goias (GO05), and Espirito Santo (ES-504) in 2017, and Rio de Janeiro (RJ 155) in 2019. These isolates have genomic differences mainly distributed in non-structural proteins. We compared the isolates' rates of infection in mammal and mosquito cells and neurovirulence in adult mice. RJ 155 and PR4408 YFV isolates exhibited higher infectivity in mammalian cells and neurovirulence in mice. In mosquito Aag2 cells, GO05 and PR4408 displayed the lowest proliferation rates. These results suggest that RJ 155 and PR4408 YFV isolates carry some genomic markers that increase infectivity in mammal hosts. From this characterization, it is possible to contribute to discovering new molecular markers for the virulence of YFV.

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