Journal
FRONTIERS IN MICROBIOLOGY
Volume 12, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2021.747868
Keywords
B. bruxellensis; light intensity; oxidative stress; ROS; p-coumaric acid
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Funding
- ANID/CONICYT FONDECYT Iniciacion [11180979]
- POSTDOC Universidad de Santiago de Chile [DICYT 081871GM]
- Millennium Nucleus for Fungal Integrative and Synthetic Biology [NC120043]
- Dicyt-USACH
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The study explores the growth of Brettanomyces bruxellensis under the stress of p-coumaric acid and different light intensities. The yeast adapts to these stress factors and manages to grow, with a higher expression of stress-related genes under lower light intensity indicating a protective effect.
Brettanomyces bruxellensis is considered the most significant contaminant yeast in the wine industry since it causes a deterioration in the organoleptic properties of the wine and significant economic losses. This deterioration is due to the production of volatile phenols from hydroxycinnamic acids. These compounds possess antimicrobial properties; however, B. bruxellensis can resist this effect because it metabolizes them into less toxic ones. Recent studies have reported that B. bruxellensis grows under different stress conditions, including p-coumaric acid (pCA) but effective methods for its control have not been found yet. Since that in other yeasts, such as Saccharomyces cerevisiae, it has been described that light affects its growth, and we evaluated whether the light would have a similar effect on B. bruxellensis. The results show that at light intensities of 2,500 and 4,000 lux in the absence of pCA, B. bruxellensis LAMAP2480 does not grow in the culture medium; however, when the medium contains this acid, the yeast adapts to both factors of stress managing to grow. The expression of genes related to oxidative stress in B. bruxellensis LAMAP2480, such as SOD1, GCN4, and ESBP6, showed a higher relative expression when the yeast was exposed to 2,500 lux compared to 4,000 lux, agreeing with the growth curves. This suggests that a higher expression of the genes studied would be related to stress-protective effects by pCA.
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