Diversity, Chemical Constituents, and Biological Activities of Endophytic Fungi Isolated From Ligusticum chuanxiong Hort

The objective of this study was to evaluate the diversity of endophytic fungi of different parts of Ligusticum chuanxiong Hort (CX) and further characterize their biological activities and identify chemical compounds produced by these endophytic fungi. A total of 21 endophytic fungi were isolated and identified from CX. Penicillium oxalicum, Simplicillium sp., and Colletotrichum sp. were identified as promising strains by the color reaction. Comparing different organic extracts of the three strains, it was observed that the ethyl acetate extract of Penicillium oxalicum and Simplicillium sp. and the n-butanol extract of Colletotrichum sp. showed significant antioxidant and antibacterial activities. The ethyl acetate extracts of Penicillium oxalicum had outstanding antioxidant and antibacterial effects, and its radical scavenging rates for ABTS and DPPH were 98.43 +/- 0.006% and 90.11 +/- 0.032%, respectively. At the same time, their IC50 values were only 0.18 +/- 0.02 mg/mL and 0.04 +/- 0.003 mg/mL. The ethyl acetate extract of Penicillium oxalicum showed MIC value of only 0.5 mg/mL against Escherichia coli and Staphylococcus aureus. By liquid chromatography-mass spectrometry (LC-MS), we found that Penicillium oxalicum could produce many high-value polyphenols, such as hesperidin (36.06 mu mol/g), ferulic acid (1.17 mu mol/g), and alternariol (12.64 mu mol/g), which can be a potential resource for the pharmaceutical industry. In conclusion, these results increase the diversity of CX endophytic fungi and the antioxidant and antibacterial activities of their secondary metabolites.

Automated summary

This study evaluated the diversity of endophytic fungi in different parts of Ligusticum chuanxiong Hort and found that some of the isolated fungi showed significant antioxidant and antibacterial activities, potentially producing valuable polyphenols. Detailed analysis of the ethyl acetate extract of Penicillium oxalicum demonstrated outstanding performance in scavenging free radicals and inhibiting bacteria.