Journal
FRONTIERS IN MICROBIOLOGY
Volume 12, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2021.652477
Keywords
Sinorhizobium meliloti; homeoviscous adaptation; temperature change; plant-bacteria interaction; outer and inner membrane
Categories
Funding
- PIP CONICET [11220200103025CO, 11220150100232CO]
- SeCyT-UNRC [161/126]
- PICT [11220150100232CO, 4162/18, 4420/2018]
- SeCyT-UNC [33620180100680CB]
- [1065/15]
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The study reveals that the fluidity of Sinorhizobium meliloti membranes changes rapidly under cyclic temperature fluctuations, with different behaviors observed in the outer and inner membranes. After incubation at 40 degrees Celsius, cells were unable to recover the preheating membrane levels at 10 degrees Celsius, leading to delayed formation of structures in Medicago sativa infected with S. meliloti.
Here, we estimate fast changes in the fluidity of Sinorhizobium meliloti membranes submitted to cyclic temperature changes (10 degrees C-40 degrees C-10 degrees C) by monitoring the fluorescence polarization (P) of DPH and TMA-DPH of the whole cell (WC) as well as in its outer (OM) and inner (IM) membranes. Additionally, the long-term response to thermal changes is demonstrated through the dynamics of the phospholipid and fatty acid composition in each membrane. This allowed membrane homeoviscous adaptation by the return to optimal fluidity levels as measured by the PDPH/TMA-DPH in WC, OM, IM, and multilamellar vesicles of lipids extracted from OM and IM. Due to probe-partitioning preferences and membranes' compositional characteristics, DPH and TMA-DPH exhibit different behaviors in IM and OM. The rapid effect of cyclic temperature changes on the P was the opposite in both membranes with the IM being the one that exhibited the thermal behavior expected for lipid bilayers. Interestingly, only after the incubation at 40 degrees C, cells were unable to recover the membrane preheating P levels when cooled up to 10 degrees C. Solely in this condition, the formation of threads and nodular structures in Medicago sativa infected with S. meliloti were delayed, indicating that the symbiotic interaction was partially altered but not halted.
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