Journal
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
Volume 11, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fcimb.2021.756854
Keywords
gd T cells; Th1/Th17 cytokines; cytotoxic and immune markers; latent tuberculosis; diabetes mellitus; pre-diabetes
Categories
Funding
- DBT-RA Program in Biotechnology and Life Sciences
- Division of Intramural Research, National Institute of Allergy and Infectious Diseases (NIAID), NIH
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In latent tuberculosis, individuals with diabetes mellitus or pre-diabetes, as well as non-diabetes comorbidities, show significantly reduced expression of cytokines, cytotoxic factors, and immune markers in gamma-delta T cells upon stimulation with specific antigens.
Antigen-specific gamma-delta (gamma delta) T cells are important in exhibiting anti-mycobacterial immunity, but their role in latent tuberculosis (LTB) with diabetes mellitus (DM) or pre-DM (PDM) and non-DM comorbidities have not been studied. Thus, we have studied the baseline, mycobacterial (PPD, WCL), and positive control antigen-stimulated gamma delta T cells expressing Th1 (IFN gamma, TNF alpha, IL-2) and Th17 (IL-17A, IL-17F, IL-22) cytokine as well as cytotoxic (perforin [PFN], granzyme [GZE B], granulysin [GNLSN]) and immune (GMCSF, PD-1, CD69) markers in LTB (DM, PDM, NDM) comorbidities by flow cytometry. In the unstimulated (UNS) condition, we did not observe any significant difference in the frequencies of gamma delta T cells expressing Th1 and Th17 cytokine, cytotoxic, and immune markers. In contrast, upon PPD antigen stimulation, the frequencies of gamma delta T cells expressing Th1 (IFN gamma, TNF alpha) and Th17 (IL-17F, IL-22) cytokine, cytotoxic (PFN, GZE B, GNLSN), and immune (CD69) markers were significantly diminished in LTB DM and/or PDM individuals compared to LTB NDM individuals. Similarly, upon WCL antigen stimulation, the frequencies of gamma delta T cells expressing Th1 (TNF alpha) and Th17 (IL-17A, IL-22) cytokine, cytotoxic (PFN), and immune (PD-1, CD69) markers were significantly diminished in LTB DM and/or PDM individuals compared to LTB NDM individuals. Finally, upon P/I stimulation we did not observe any significant difference in the gamma delta T cell frequencies expressing cytokine, cytotoxic, and immune markers between the study populations. The culture supernatant levels of IFN gamma, TNF alpha, and IL-17A cytokines were significantly increased in LTB DM and PDM after stimulation with Mtb antigens compared to LTB NDM individuals. Therefore, diminished gamma delta T cells expressing cytokine, cytotoxic, and other immune markers and elevated levels of cytokines in the supernatants is a characteristic feature of LTB PDM/DM co-morbidities.
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