Journal
ELIFE
Volume 11, Issue -, Pages -Publisher
eLIFE SCIENCES PUBL LTD
DOI: 10.7554/eLife.74090
Keywords
Rap1; Rif2; Ku complex; telomere; telomerase; tlc1-tm; S; cerevisiae
Categories
Funding
- CONACYT scholarship
- EMBO Short-Term Fellowship
- Carl Trygger Foundation
- Erik Philip-Sorensen Foundation
- Royal Physiographic Society in Lund
- Vidi grant from the Netherlands Organization for Scientific Research
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This study reveals that disruption of Rap1 at telomeres leads to defects in telomere length regulation and capping, while tlc1-tm cells grow similarly to wild-type cells. Rif2 and the Ku complex work in parallel to prevent tlc1-tm telomere degradation, highlighting the redundant mechanisms that may explain the rapid evolution of telomere components in budding yeast species.
Rap1 is the main protein that binds double-stranded telomeric DNA in Saccharomyces cerevisiae. Examination of the telomere functions of Rap1 is complicated by the fact that it also acts as a transcriptional regulator of hundreds of genes and is encoded by an essential gene. In this study, we disrupt Rap1 telomere association by expressing a mutant telomerase RNA subunit (tlc1-tm) that introduces mutant telomeric repeats. tlc1-tm cells grow similar to wild-type cells, although depletion of Rap1 at telomeres causes defects in telomere length regulation and telomere capping. Rif2 is a protein normally recruited to telomeres by Rap1, but we show that Rif2 can still associate with Rap1-depleted tlc1-tm telomeres, and that this association is required to inhibit telomere degradation by the MRX complex. Rif2 and the Ku complex work in parallel to prevent tlc1-tm telomere degradation; tlc1-tm cells lacking Rif2 and the Ku complex are inviable. The partially redundant mechanisms may explain the rapid evolution of telomere components in budding yeast species.
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