Journal
ELIFE
Volume 11, Issue -, Pages -Publisher
eLIFE SCIENCES PUBL LTD
DOI: 10.7554/eLife.70510
Keywords
locus coeruleus; pupil diameter; brain state; Mouse
Categories
Funding
- National Institute of Neurological Disorders and Stroke [R01NS112200, R01NS107355]
- University of California, Riverside
- University of California
- Esther A and Joseph Klingenstein Fund
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Pupil diameter is often used as a noninvasive measure of locus coeruleus (LC) activity. However, there is substantial variability in the relationship between pupil diameter and LC activity, limiting its accuracy in predicting LC activity. Additionally, pupil responses to optical stimulation in the LC show session-to-session fluctuations, and these variations are correlated with decision bias-related behavioral variables.
Pupil diameter is often treated as a noninvasive readout of activity in the locus coeruleus (LC). However, how accurately it can be used to index LC activity is not known. To address this question, we established a graded relationship between pupil size changes and LC spiking activity in mice, where pupil dilation increased monotonically with the number of LC spikes. However, this relationship exists with substantial variability such that pupil diameter can only be used to accurately predict a small fraction of LC activity on a moment-by-moment basis. In addition, pupil exhibited large session-to-session fluctuations in response to identical optical stimulation in the LC. The variations in the pupil-LC relationship were strongly correlated with decision bias-related behavioral variables. Together, our data show that substantial variability exists in an overall graded relationship between pupil diameter and LC activity, and further suggest that the pupil-LC relationship is dynamically modulated by brain states, supporting and extending our previous findings (Yang et al., 2021).
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