4.8 Article

Discovery of coordinately regulated pathways that provide innate protection against interbacterial antagonism

Journal

ELIFE
Volume 11, Issue -, Pages -

Publisher

eLIFE SCIENCES PUBL LTD
DOI: 10.7554/eLife.74658

Keywords

interbacterial antagonism; innate immunity; Tn-seq; Pseudomonas aeruginosa; toxin; phospholipase; Other

Categories

Funding

  1. NIH [AI080609, DK089507, R01AI136979]
  2. Cystic Fibrosis Foundation [SINGH19R0]
  3. Office of the Director, National Institutes of Health [S10OD026741]

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Pseudomonas aeruginosa shows a coordinated defense against bacterial threats by activating conserved interbacterial antagonism resistance clusters, similar to eukaryotic innate immunity.
Bacterial survival is fraught with antagonism, including that deriving from viruses and competing bacterial cells. It is now appreciated that bacteria mount complex antiviral responses; however, whether a coordinated defense against bacterial threats is undertaken is not well understood. Previously, we showed that Pseudomonas aeruginosa possess a danger-sensing pathway that is a critical fitness determinant during competition against other bacteria. Here, we conducted genome-wide screens in P. aeruginosa that reveal three conserved and widespread interbacterial antagonism resistance clusters (arc1-3). We find that although arc1-3 are coordinately activated by the Gac/Rsm danger-sensing system, they function independently and provide idiosyncratic defense capabilities, distinguishing them from general stress response pathways. Our findings demonstrate that Arc3 family proteins provide specific protection against phospholipase toxins by preventing the accumulation of lysophospholipids in a manner distinct from previously characterized membrane repair systems. These findings liken the response of P. aeruginosa to bacterial threats to that of eukaryotic innate immunity, wherein threat detection leads to the activation of specialized defense systems.

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