Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 84, Issue -, Pages 289-294Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2015.12.045
Keywords
Mung bean; Alkali-extractable polysaccharide; Antioxidant
Funding
- Hong Kong, Macao and Taiwan Science & Technology Cooperation Program of China [2014DFT30280, 2013DFT3050]
- Special Fund for Agro-scientific Research in the Public Interest [201303069]
- earmarked fund for China Agriculture Research System Modern Agro-industry Technology [CYTX-014]
- Agricultural Science and Technology Program for Innovation Team on Identification and excavation of Elite Crop Germplasm, CAAS
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Alkali-extractable polysaccharides from the seeds of mung beans and two polysaccharide sub-fractions (MAP-1 and MAP-2) were isolated and purified by anion-exchange and gel filtration chromatography. The average molecular weights (Mws) of MAP-1 and MAP-2 were 94.2 kDa and 60.4 kDa, respectively. Monosaccharide component analysis indicated that MAP-1 was composed of Rha, Ara, Glu, Gal, and GalA in a molar ratio of 1.1:0.4:0.7:0.5:0.3. MAP-2 consisted of Xyl, Rha, Gal, Glu and GalA with a relative molar ratio of 0.4:1.4:1.6:0.5:0.2. Antioxidant assays indicated that both MAP-I and MAP-2 exhibit significant antioxidant activity in a dose-dependent manner. An in vitro study further showed that MAP-1 and MAP-2 were both able to stimulate the production of secretory molecules (NO, TNF-alpha and IL-6) by RAW 264.7 murine macrophages in a concentration-dependent manner. These findings suggest that the polysaccharides isolated in our study have immunoregulatory effects on macrophages and can be used as a beneficial health food. (C) 2015 Published by Elsevier B.V.
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