Journal
PROTEIN & CELL
Volume 13, Issue 10, Pages 760-777Publisher
OXFORD UNIV PRESS
DOI: 10.1007/s13238-021-00895-y
Keywords
nuclear pore complex; cryo-EM; Xenopus laevis; AlphaFold2; nuclear ring; cytoplasmic ring
Categories
Funding
- Ministry of Science and Technology of China [2017YFA0504700, 2016YFA0500201]
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDB 37040102]
- National Natural Science Foundation of China [31830020, 31520103906, 31430051]
- National Science Fund for Distinguished Young Scholars [31925026]
- National Key Research and Development Program of China [2016YFA0100501, 2018YFA0901102]
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The nuclear pore complex (NPC) is a large protein complex in eukaryotes that regulates the transport of molecules between the nucleus and cytoplasm. By using cryo-electron microscopy and AlphaFold2, researchers determined the structure of the outer rings of the Xenopus laevis NPC and identified the composition and interactions of its components. This detailed structural information represents a significant advancement in understanding the assembly of the NPC.
The nuclear pore complex (NPC), one of the largest protein complexes in eukaryotes, serves as a physical gate to regulate nucleocytoplasmic transport. Here, we determined the 8 angstrom resolution cryo-electron microscopic (cryo-EM) structure of the outer rings containing nuclear ring (NR) and cytoplasmic ring (CR) from the Xenopus laevis NPC, with local resolutions reaching 4.9 angstrom. With the aid of AlphaFold2, we managed to build a pseudoatomic model of the outer rings, including the Y complexes and flanking components. In this most comprehensive and accurate model of outer rings to date, the almost complete Y complex structure exhibits much tighter interaction in the hub region. In addition to two copies of Y complexes, each asymmetric subunit in CR contains five copies of Nup358, two copies of the Nup214 complex, two copies of Nup205 and one copy of newly identified Nup93, while that in NR contains one copy of Nup205, one copy of ELYS and one copy of Nup93. These in-depth structural features represent a great advance in understanding the assembly of NPCs.
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