Journal
POLYMERS
Volume 14, Issue 2, Pages -Publisher
MDPI
DOI: 10.3390/polym14020344
Keywords
nanotopography; iron oxide nanoparticles; DNA; hTERT-transduced mesenchymal stem cells; osteogenesis; adipogenesis; chondrogenesis
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Funding
- Russian Scientific Foundation [21-74-10034]
- Russian Science Foundation [21-74-10034] Funding Source: Russian Science Foundation
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This study demonstrates that substrates modified with magnetic nanoparticles and DNA can induce the differentiation of human adipose-derived MSCs into chondrocytes and osteoblasts. An increase in the concentration of magnetic nanoparticles leads to reduced cell adhesion and increased surface roughness.
Mesenchymal stem cells (MSCs) have extensive pluripotent potential to differentiate into various cell types, and thus they are an important tool for regenerative medicine and biomedical research. In this work, the differentiation of hTERT-transduced adipose-derived MSCs (hMSCs) into chondrocytes, adipocytes and osteoblasts on substrates with nanotopography generated by magnetic iron oxide nanoparticles (MNPs) and DNA was investigated. Citrate-stabilized MNPs were synthesized by the chemical co-precipitation method and sized around 10 nm according to microscopy studies. It was shown that MNPs@DNA coatings induced chondrogenesis and osteogenesis in hTERT-transduced MSCs. The cells had normal morphology and distribution of actin filaments. An increase in the concentration of magnetic nanoparticles resulted in a higher surface roughness and reduced the adhesion of cells to the substrate. A glass substrate modified with magnetic nanoparticles and DNA induced active chondrogenesis of hTERT-transduced MSC in a twice-diluted differentiation-inducing growth medium, suggesting the possible use of nanostructured MNPs@DNA coatings to obtain differentiated cells at a reduced level of growth factors.
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