Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 89, Issue -, Pages 376-388Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2016.05.002
Keywords
Bletilla striata polysaccharide; Reactive oxygen species; Inflammation
Funding
- National Natural Science Foundation [81173527, 81202900]
- National Scientific and Technological Major Project for Significant New Drugs Creation [2012ZX09502001-004]
- Natural Science Foundation of Jiangsu Province [BK2012359]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
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In the current study, we analyzed the functions and mechanisms of Bletilla striata polysaccharide b (BSPb) against Angiotensin II (Ang II)-induced oxidative stress and inflammation in human mesangial cells (HMCs). It was found that BSPb could inhibit generation of Ang II-induced reactive oxygen species (ROS) and activation of proinflammatory cytokines interleukin 6 (IL-6) and tumor necrosis factor a (TNF-alpha) in a dose-dependent manner. Further studies revealed that BSPb effectively blocked upregulation of NADPH oxidase 4 (NOX4). Moreover, knockdown of NOX4 significantly impaired the anti-oxidative function of BSPb. In addition, BSPb decreased overexpression of Toll-like receptor 2 (TLR2) induced by Ang II. Blocking TLR2 expression impaired the anti-inflammatory effects of BSPb. In conclusion, BSPb was found to possess anti-oxidative stress and anti-inflammatory functions against Ang II-induced ROS generation and proinflammatory cytokines activation. The NOX4 and TLR2 pathways played important roles in the biological effects mediated by BSPb. (C) 2016 Elsevier B.V. All rights reserved.
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