Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 86, Issue -, Pages 848-856Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2016.02.015
Keywords
Chitosan nanoparticles; Anti-inflammatory effects; Caco-2 cell
Funding
- Zhejiang Chinese Medical University Foundation [2011ZZ13]
- Zhejiang A&F University of Scientific Research [2012FR097]
- Zhejiang Provincial Science and Technology Council [2014C37007]
- National Natural Science Foundation of China [31501985]
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Chitosan nanoparticles (CNP), an extensively oral-administered drug carrier, was investigated for the anti-inflammatory effects on LPS-inflamed Caco-2 cells and the relate mechanisms. CNP could alleviate the decrease of transepithelial electrical resistance (TEER) induced by LPS in Caco-2 monolayer, and significantly inhibit LPS-induced production of TNF-alpha, MIF, IL-8 and MCP-1 in a dose-dependent manner. PCR array assay revealed that CNP down-regulated the mRNA expression levels of TLR4 in LPS-inflamed Caco-2 cells. CNP was further showed to reduce cytoplasmic I kappa B-alpha degradation and nuclear NF-kappa B p65 levels in LPS-inflamed Caco-2 cells. These results suggested that CNP suppressed LPS-induced inflammatory response by decreasing permeability of intestinal epithelial monolayer and secretion of pro-inflammatory cytokine in Caco-2 cells, which were partially mediated by NF-kappa B signaling pathway. (C) 2016 Elsevier B.V. All rights reserved.
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