Journal
POLYMERS
Volume 13, Issue 23, Pages -Publisher
MDPI
DOI: 10.3390/polym13234257
Keywords
poly(3-hydroxybutyrate); bioplastics; biodegradation; screening; Microbulbifer genus
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Funding
- National Research Foundation of Korea (NRF) [NRF-2019R1F1A1058805, NRF-2019M3E6A1103979]
- Research Program to solve the social issues of the NRF - Ministry of Science and ICT [2017M3A9E4077234]
- R&D Program of MOTIE/KEIT [20009508, 20014350]
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The study identified Microbulbifer sp. SOL66 as a highly efficient strain in degrading PHB, with the ability to completely degrade PHB within a short period under experimental conditions. Additionally, the addition of xylose and ammonium sulfate can enhance its degradation activity.
Having the advantage of eco-friendly decomposition, bioplastics could be used to replace petroleum-based plastics. In particular, poly(3-hydroxybutyrate) (PHB) is one of the most commercialized bioplastics, however, necessitating the introduction of PHB-degrading bacteria for its effective disposal. In this study, Microbulbifer sp. SOL66 (94.18% 16S rRNA with similarity to Microbulbifer hydrolyticus) demonstrated the highest degradation activity among five newly screened Microbulbifer genus strains. Microbulbifer sp. SOL66 showed a rapid degradation yield, reaching 98% in 4 days, as monitored by laboratory scale, gas chromatography-mass spectrometry, scanning electron microscopy, gel permeation chromatography, and Fourier transform infrared spectroscopy. The PHB film was completely degraded within 7 days at 37 degrees C in the presence of 3% NaCl. When 1% xylose and 0.4% ammonium sulfate were added, the degradation activity increased by 17% and 24%, respectively. In addition, this strain showed biodegradability on pellets of poly(3-hydroxybutyrate-co-4-hydroxybutyrate), as confirmed by weight loss and physical property changes. We confirmed that Microbulbifer sp. SOL66 has a great ability to degrade PHB, and has rarely been reported to date.
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