4.7 Article

One-Pot Synthesis of Amphiphilic Biopolymers from Oxidized Alginate and Self-Assembly as a Carrier for Sustained Release of Hydrophobic Drugs

Journal

POLYMERS
Volume 14, Issue 4, Pages -

Publisher

MDPI
DOI: 10.3390/polym14040694

Keywords

amphiphilic conjugates; sodium alginate; nanocarrier; self-assembly; sustained release

Funding

  1. National Natural Science Foundation of China [51963009]
  2. Key Research and Development Project of Hainan Province [ZDYF2019018]
  3. Natural Science Foundation of Hainan Province [220MS035]
  4. Scientific Research Fund of Jiangxi Provincial Education Department [GJJ201502]
  5. Open Fund for Innovation and Entrepreneurship of College Students of Hainan Normal University [HSRS21-057]

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In this study, an organic solvent-free, eco-friendly and efficient approach for the preparation of amphiphilic conjugates (Ugi-OSAOcT) was developed by grafting octylamine to oxidized sodium alginate. The conjugates self-assembled into stable and spherical micelles and successfully loaded the poorly water-soluble drug ibuprofen. The loaded micelles exhibited sustained and pH-responsive drug release behavior.
In this paper, we developed an organic solvent-free, eco-friendly, simple and efficient one-pot approach for the preparation of amphiphilic conjugates (Ugi-OSAOcT) by grafting octylamine (OCA) to oxidized sodium alginate (OSA). The optimum reaction parameters that were obtained based on the degree of substitution (DS) of Ugi-OSAOcT were a reaction time of 12 h, a reaction temperature of 25 degrees C and a molar ratio of 1:2.4:3:3.3 (OSA:OCA:HAc:TOSMIC), respectively. The chemical structure and composition were characterized by Fourier transform infrared spectroscopy (FTIR), H-1 nuclear magnetic resonance (H-1 NMR), X-ray diffraction (XRD), thermogravimetry analyser (TGA), gel permeation chromatography (GPC) and elemental analysis (EA). It was found that the Ugi-OSAOcT conjugates with a CMC value in the range of 0.30-0.085 mg/mL could self-assemble into stable and spherical micelles with a particle size of 135.7 +/- 2.4-196.5 +/- 3.8 nm and negative surface potentials of -32.8 +/- 0.4--38.2 +/- 0.8 mV. Furthermore, ibuprofen (IBU), which served as a model poorly water-soluble drug, was successfully incorporated into the Ugi-OSAOcT micelles by dialysis method. The drug loading capacity (%DL) and encapsulation efficiency (%EE) of the IBU-loaded Ugi-OSAOcT micelles (IBU/Ugi-OSAOcT = 3:10) reached as much as 10.9 +/- 0.4-14.6 +/- 0.3% and 40.8 +/- 1.6-57.2 +/- 1.3%, respectively. The in vitro release study demonstrated that the IBU-loaded micelles had a sustained and pH-responsive drug release behavior. In addition, the DS of the hydrophobic segment on an OSA backbone was demonstrated to have an important effect on IBU loading and drug release behavior. Finally, the in vitro cytotoxicity assay demonstrated that the Ugi-OSAOcT conjugates exhibited no significant cytotoxicity against RAW 264.7 cells up to 1000 mu g/mL. Therefore, the amphiphilic Ugi-OSAOcT conjugates synthesized by the green method exhibited great potential to load hydrophobic drugs, acting as a promising nanocarrier capable of responding to pH for sustained release of hydrophobic drugs.

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