Laccase-Mediator System Using a Natural Mediator as a Whitening Agent for the Decolorization of Melanin

In this study, a laccase-mediator system (LMS) using a natural mediator was developed as a whitening agent for melanin decolorization. Seven natural mediators were used to replace synthetic mediators and successfully overcome the low redox potential of laccase and limited access of melanin to the active site of laccase. The melanin decolorization activity of laccases from Trametes versicolor (lacT) and Myceliophthora thermophila (lacM) was significantly enhanced using natural mediators including acetosyringone, syringaldehyde, and acetovanillone, which showed low cytotoxicity. The methoxy and ketone groups of natural mediators play an important role in melanin decolorization. The specificity constants of lacT and lacM for melanin decolorization were enhanced by 247 and 334, respectively, when acetosyringone was used as a mediator. LMS using lacM and acetosyringone could also decolorize the melanin present in the cellulose hydrogel film, which mimics the skin condition. Furthermore, LMS could decolorize not only synthetic eumelanin analogs prepared by the oxidation of tyrosine but also natural melanin produced by melanoma cells.

Automated summary

This study developed a laccase-mediator system using natural mediators for melanin decolorization, effectively overcoming issues such as the low redox potential of laccase and limited access of melanin to the active site. Natural mediators with methoxy and ketone groups played a crucial role in melanin decolorization, and using acetosyringone as a mediator significantly enhanced the specificity constants of laccases. The laccase-mediator system could decolorize synthetic eumelanin analogs and natural melanin produced by melanoma cells, showing promising potential for skin whitening applications.