Evaluation of enzyme treatment conditions on extraction of anthocyanins from Prunus nepalensis L.

The study was designed to investigate the effect of enzyme assisted extraction of anthocyanins from Sohiong fruit (Prunus nepalensis) under varied time, temperature and treatment conditions. Highest anthocyanins yield was obtained by coupling enzymatic treatment along with solvent extraction simultaneously. Additionally, effect of enzyme type, enzyme concentration, reaction time and temperature were evaluated subsequently in following experiments. Cellulase treatment (10% E/S) for 180 min at 4 degrees C exhibited highest yield of 984.40 +/- 3.84 mg OG/100 g dm which accounts to 14.61% higher yield when compared to conventional method (858.84 +/- 6.88 mg OG/100 g dm). The study provides an economical alternative for commercial extraction of anthocyanins from Sohiong fruit which can be used as a colourant for various food and other products and owing to its antioxidizing properties can be effective for the prevention and treatment of diseases. (C) 2016 Elsevier B.V. All rights reserved.