Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 86, Issue -, Pages 277-281Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2016.01.073
Keywords
Porcine circovirus 2 (PCV2); ORF; Antibody
Funding
- Jilin Province Science and Technology Development Projects [20140101123JC]
- Fundamental Research Funds of Jilin University
- Science and Technology Research Program during the Twelfth Five-year Plan Period of Jilin Educational Committee
- Graduate Innovation Fund of Jilin University
- Program for Changjiang Scholars and Innovative Research Team in University [IRT1248]
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Rep and ORF3 proteins are important functional proteins of porcine circovirus 2 (PCV2). Here, Rep and ORF3 genes were cloned, expressed and used to raise polyclonal antibodies. The result showed the recombinant plasmids of Rep and ORF3 genes constructed in this study were expressed efficiently in the prokaryotic system, and the recombinant proteins had antigenicity and immunogenicity. Furthermore, reactivity and specificity of the antiserums were characterized by western blot and indirect immunofluorescent assays. The results elucidated that polyclonal antiserum prepared with Rep or ORF3 had good reactivity and specificity against PCV2, or the Rep and ORF3 expressed in PK-15 cells, respectively. The Rep protein is promising for PCV2 antibody and vaccine development. These results will be helpful for further studies focusing on pathogenesis of PCV2 and serology diagnostic test or vaccine development against PCV2. (C) 2016 Elsevier B.V. All rights reserved.
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