Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 85, Issue -, Pages 258-270Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2015.12.077
Keywords
Box-Behnken design; Solid lipid nanoparticles; SLN; HET-CAM; Levofloxacin; Ocular drug delivery; Optimization; Antibacterial activity
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The aim of the present study was to develop and optimize levofloxacin loaded solid lipid nanoparticles for the treatment of conjunctivitis. Box-Behnken experimental design was applied for optimization of solid lipid nanoparticles. The independent variables were stearic acid as lipid (X-1), Tween 80 as surfactant (X-2) and sodium deoxycholate as co-surfactant (X-3) while particle size (Y-1) and entrapment efficiency (Y-2) were the dependent variables. Further in vitro release and antibacterial activity in vitro were also performed. The optimized formulation of levofloxacin provides particle size of 237.82 nm and showed 78.71% entrapment efficiency and achieved flux 0.2493 mu g/cm(2)/h across excised goat cornea. In vitro release study showed prolonged drug release from the optimized formulation following Korsmeyer-Peppas model. Antimicrobial study revealed that the developed formulation possesses antibacterial activity against Staphylococcus aureus, and Escherichia coli equivalent to marketed eye drops. HET-CAM test demonstrated that optimized formulation was found to be non-irritant and safe for topical ophthalmic use. Our results concluded that solid lipid nanoparticles are an efficient carrier for ocular delivery of levofloxacin and other drugs. (C) 2015 Elsevier B.V. All rights reserved.
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