Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 93, Issue -, Pages 1457-1464Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2015.12.083
Keywords
Carboxylmethyl cellulose/Zn doped; nano-hydroxyapatite/ascorbic acid; miR-15b; Mesenchymal stem cells
Funding
- Council of Scientific and Industrial Research, India [37(1574)/12/EMR-II, 60(0110)/13/EMR-II]
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The present study was aimed to synthesize and characterize a bio-composite scaffold containing carboxymethyl cellulose (CMC), zinc doped nano-hydroxyapatite (Zn-nHAp) and ascorbic acid (AC) for bone tissue engineering applications. The fabricated bio-composite scaffold was characterized by SEM, FT-IR and XRD analyses. The ability of scaffold along with a bioactive molecule, microRNA-15b (miR-15b) for osteo-differentiation at cellular and molecular levels was determined using mouse mesenchymal stem cells (mMSCs). miR-15b acts as posttranscriptional gene regulator and regulates osteoblast differentiation. The scaffold and miR-15b were able to promote osteoblast differentiation; when these treatments were combined together on mMSCs, there was an additive effect on promotion of osteoblast differentiation. Thus, it appears that the combination of CMC/Zn-nHAp/AC scaffold with miR-15b would provide more efficient strategy for treating bone related defects and bone regeneration. (C) 2015 Elsevier B.V. All rights reserved.
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