Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 85, Issue -, Pages 277-284Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2015.12.046
Keywords
Polysaccharides; Ligusticum chuanxiong; Ultrasonic-assisted extraction; Antioxidant; Anticancer
Funding
- Macao Science and Technology Development Fund [102/2012/A3]
- University of Macau [MYRG2014-00033-ICMS-QRCM, MYRG2014-00051-ICMS-QRCM, MYRG107(Y1-L3)-ICMS13-HCW, MYRG2015-00081-ICMS-QRCM]
- National Natural Science Foundation of China [81403120]
Ask authors/readers for more resources
Ultrasonic-assisted extraction technology was employed to prepare Ligusticum chuanxiong Hort polysaccharide. Single factor test and orthogonal experimental design were used to optimize the extraction conditions. The results showed that the optimal extraction conditions consisted of ultrasonic temperature of 80 degrees C, ultrasonic time of 40 min and water to raw material ratio of 30 mL/g. Three novel polysaccharides fractions, LCX0, LCX1 and LCX2, were isolated and purified from the crude polysaccharides using DEAE-52 cellulose and Sephadex G-100 column chromatography. The molecular weight and monosaccharide composition of three LCX polysaccharides fractions were analyzed with gel permeation chromatography (GPC) and HPLC analysis, respectively. Furthermore, the antioxidant and in vitro anticancer activities of the polysaccharides were investigated. Compared with LCX0, LCX2 and LCX1 showed relative higher antioxidant activity and inhibitory activity to the growth of HepG2, SMMC7721, A549 and HCT-116 cells. It is suggested that the novel polysaccharides from rhizome of L. chuanxiong could be promising bioactive macromolecules for biomedical use. (C) 2016 Published by Elsevier B.V.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available