Journal
G3-GENES GENOMES GENETICS
Volume 12, Issue 1, Pages -Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/g3journal/jkab376
Keywords
long-reads; PacBio; optical mapping; low-coverage whole-genome sequencing; sex determination; Y-autosomal fusion; flatfish
Categories
Funding
- Canada 150 Sequencing Initiative (Canseq150)
- CFI
- Genome Canada Technology Platform grants
- National Sciences and Engineering Research Council of Canada (NSERC strategic partnership program) grant
- Canadian Research Chair in Genomics and Conservation of Aquatic Resources
- Ressources Aquatiques Quebec (RAQ)
Ask authors/readers for more resources
A chromosome-level reference genome for Greenland Halibut was successfully assembled using single-molecule sequencing and chromatin conformation capture sequencing data. The study revealed that Greenland Halibut possesses a male heterogametic XY system for sex determination, similar to other teleost fishes, and identified several candidate genes potentially involved in this process. Additionally, the study suggested a putative Y-autosomal fusion may be associated with reduced recombination during the early steps of sex chromosome evolution in flatfishes.
Despite the commercial importance of Greenland Halibut (Reinhardtius hippoglossoides), important gaps still persist in our knowledge of this species, including its reproductive biology and sex determination mechanism. Here, we combined single-molecule sequencing of long reads (Pacific Sciences) with chromatin conformation capture sequencing (Hi-C) data to assemble the first chromosome-level reference genome for this species. The high-quality assembly encompassed more than 598 Megabases (Mb) assigned to 1594 scaffolds (scaffold N50 = 25Mb) with 96% of its total length distributed among 24 chromosomes. Investigation of the syntenic relationship with other economically important flatfish species revealed a high conservation of synteny blocks among members of this phylogenetic clade. Sex determination analysis revealed that similar to other teleost fishes, flatfishes also exhibit a high level of plasticity and turnover in sex determination mechanisms. A low-coverage whole-genome sequence analysis of 198 individuals revealed that Greenland Halibut possesses a male heterogametic XY system and several putative candidate genes implied in the sex determination of this species. Our study also suggests for the first time in flatfishes that a putative Y-autosomal fusion could be associated with a reduction of recombination typical of the early steps of sex chromosome evolution.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available