Journal
CELL REPORTS
Volume 37, Issue 5, Pages -Publisher
CELL PRESS
DOI: 10.1016/j.celrep.2021.109929
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Funding
- Argonne National Laboratory (ANL), Structural Biology Center (SBC) at the Advanced Photon Source (APS), under U.S. Department of Energy, Office of Biological and Environmental Research [DE-AC02-06CH11357]
- National Institutes of Health (NIH)/National Institute of Allergy and Infectious Diseases (NIAID) [R01-AI127521]
- Intramural Research Program of the Vaccine Research Center (VRC), NIAID, NIH
- NIH [HHSN272201700036I, 75N93019F00132, 5494549, U01 AI149644, HHSN261200800001E, 17x198]
- Leidos Biomedical Research
- Undergraduate Scholarship Program, Office of Intramural Training and Education, Office of the Director, NIH
- US Department of Defense, Defense Advanced Research Projects Agency (DARPA) - Pandemic Prevention Platform [D18AC00002]
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This study developed stabilized stem (SS) antigens by removing the S1 subunit from the Middle East respiratory syndrome (MERS)-CoV spike (S) glycoprotein using structure-guided protein engineering. Vaccination with MERS SS elicited cross-reactive b-CoV antibody responses and protected mice against lethal MERS-CoV challenge. The discovery of a panel of cross-reactive monoclonal antibodies, among which IgG22 showed high affinity binding to both MERS-CoV and severe acute respiratory syndrome (SARS)-CoV-2 S proteins, indicates the potential for cross-reactive CoV antibodies.
Current coronavirus (CoV) vaccines primarily target immunodominant epitopes in the S1 subunit, which are poorly conserved and susceptible to escape mutations, thus threatening vaccine efficacy. Here, we use structure-guided protein engineering to remove the S1 subunit from the Middle East respiratory syndrome (MERS)-CoV spike (S) glycoprotein and develop stabilized stem (SS) antigens. Vaccination with MERS SS elicits cross-reactive b-CoV antibody responses and protects mice against lethal MERS-CoV challenge. High-throughput screening of antibody-secreting cells from MERS SS-immunized mice led to the discovery of a panel of cross-reactive monoclonal antibodies. Among them, antibody IgG22 binds with high affinity to both MERS-CoV and severe acute respiratory syndrome (SARS)-CoV-2 S proteins, and a combination of electron microscopy and crystal structures localizes the epitope to a conserved coiled-coil region in the S2 subunit. Passive transfer of IgG22 protects mice against both MERS-CoV and SARS-CoV-2 challenge. Collectively, these results provide a proof of principle for cross-reactive CoV antibodies and inform the devel
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