Journal
CELL REPORTS
Volume 37, Issue 9, Pages -Publisher
CELL PRESS
DOI: 10.1016/j.celrep.2021.110064
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Funding
- Frederick Banting and Charles Best Canada Graduate Doctoral Award (CIHR)
- Tomlinson Doctoral Fellowship (McGill)
- Cole Foundation Postdoctoral Fellowship
- NOW Vici grant [016.140.655]
- NSERC [201603808]
- McGill start-up fund
- Cancer Research Society [22344]
- CIHR [PJT-168862]
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The study found that pre-existing transcriptional and chromatin landscape differences among naive CD4(+) T cells impact their effector potential, with some cells showing a tendency towards follicular helper T cell lineage. Furthermore, the strength of TCR signal during thymic development plays a crucial role in establishing chromatin landscapes that ultimately shape the effector potential of naive CD4(+) T cells.
CD4(+) T cells have a remarkable potential to differentiate into diverse effector lineages following activation. Here, we probe the heterogeneity present among naive CD4(+) T cells before encountering their cognate antigen to ask whether their effector potential is modulated by pre-existing transcriptional and chromatin landscape differences. Single-cell RNA sequencing shows that key drivers of variability are genes involved in T cell receptor (TCR) signaling. Using CD5 expression as a readout of the strength of tonic TCR interactions with self-peptide MHC, and sorting on the ends of this self-reactivity spectrum, we find that pre-existing transcriptional differences among naive CD4(+) T cells impact follicular helper T (T-FH) cell versus non-T-FH effector lineage choice. Moreover, our data implicate TCR signal strength during thymic development in establishing differences in naive CD4(+) T cell chromatin landscapes that ultimately shape their effector potential.
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