Journal
CELL REPORTS
Volume 37, Issue 5, Pages -Publisher
CELL PRESS
DOI: 10.1016/j.celrep.2021.109914
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- European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme [678610]
- European Research Council (ERC) [678610] Funding Source: European Research Council (ERC)
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This study combines recordings of mechanosensitive currents in mechanosensory neurons with single-cell RNA sequencing to explore the role of transcriptional profiles on mechanotransduction mechanisms. The research revealed that Piezo2 is enriched in rapidly adapting neurons expressing MS currents, while Tmem120a and Tmem150c are uniformly expressed in all mechanosensory neuron subtypes.
A variety of mechanosensory neurons are involved in touch, proprioception, and pain. Many molecular components of the mechanotransduction machinery subserving these sensory modalities remain to be discovered. Here, we combine recordings of mechanosensitive (MS) currents in mechanosensory neurons with single-cell RNA sequencing. Transcriptional profiles are mapped onto previously identified sensory neuron types to identify cell-type correlates between datasets. Correlation of current signatures with single-cell transcriptomes provides a one-to-one correspondence between mechanoelectric properties and transcriptomically defined neuronal populations. Moreover, a gene-expression differential comparison provides a set of candidate genes for mechanotransduction complexes. Piezo2 is expectedly found to be enriched in rapidly adapting MS current-expressing neurons, whereas Tmem120a and Tmem150c, thought to mediate slow type MS currents, are uniformly expressed in all mechanosensory neuron subtypes. Further knockdown experiments disqualify them as mediating MS currents in sensory neurons. This dataset constitutes an open resource to explore further the cell-type-specific determinants of mechanosensory properties.
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