Journal
CELL REPORTS
Volume 37, Issue 11, Pages -Publisher
CELL PRESS
DOI: 10.1016/j.celrep.2021.110112
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Funding
- Yunnan Provincial Science and Technology Department [2019HC006]
- Kunming Science and Technology Department [2020-1-N-037]
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The developed candidate vaccine combines N protein and prefusion-full S protein, generating stronger systemic and mucosal humoral immunity through the combination of flagellin and cyclic GMP-AMP. Additionally, administering the candidate vaccine via intranasal route can enhance local immune responses in the respiratory tract and protect against lethal SARS-CoV-2 challenge.
An ideal vaccine against SARS-CoV-2 is expected to elicit broad immunity to prevent viral infection and disease, with efficient viral clearance in the upper respiratory tract (URT). Here, the N protein and prefusion-full S protein (SFLmut) are combined with flagellin (KF) and cyclic GMP-AMP (cGAMP) to generate a candidate vaccine, and this vaccine elicits stronger systemic and mucosal humoral immunity than vaccines containing other forms of the S protein. Furthermore, the candidate vaccine administered via intranasal route can enhance local immune responses in the respiratory tract. Importantly, human ACE2 transgenic mice given the candidate vaccine are protected against lethal SARS-CoV-2 challenge, with superior protection in the URT compared with that in mice immunized with an inactivated vaccine. In summary, the developed vaccine can elicit a multifaceted immune response and induce robust viral clearance in the URT, which makes it a potential vaccine for preventing disease and infection of SARS-CoV-2.
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