Journal
STEM CELL RESEARCH & THERAPY
Volume 12, Issue 1, Pages -Publisher
BMC
DOI: 10.1186/s13287-021-02587-0
Keywords
Adipose stem cells; Cell surface markers; Differentiation; Immunogenicity; Immunosuppression; Proliferation
Funding
- Business Finland
- Academy of Finland [326588, 311084, 272376, 314383, 266286]
- Competitive State Research Financing of the Expert Responsibility area of Tampere University Hospital
- Finnish Cultural Foundation [00190409]
- Tampere University Graduate School for Medicine and Life Sciences
- Helsinki University Hospital funds
- Finnish Diabetes Research Foundation
- Orion Foundation
- Paulo Foundation
- Finnish Medical Foundation
- Maud Kuistila Foundation
- Gyllenberg Foundation
- Sigrid Juselius Foundation
- Novo Nordisk Foundation [NNF17OC0027232, NNF10OC1013354]
- University of Helsinki
- Helsinki University Hospital
- Academy of Finland Centre of Excellence in Complex Disease Genetics [312073]
- Academy of Finland (AKA) [311084, 311084] Funding Source: Academy of Finland (AKA)
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This study investigated the impact of acquired weight on ASC characteristics using ASCs derived from monozygotic twin pairs. Results showed that ASCs from heavier twins had greater adipogenic potential and inflammation markers expression, but lower angiogenic potential compared to their leaner cotwins. Additionally, heavier twins exhibited stronger immunosuppressive capacity in direct cocultures.
Background Adipose stromal/stem cells (ASCs) are promising candidates for future clinical applications. ASCs have regenerative capacity, low immunogenicity, and immunomodulatory ability. The success of future cell-based therapies depends on the appropriate selection of donors. Several factors, including age, sex, and body mass index (BMI), may influence ASC characteristics. Our aim was to investigate the effect of acquired weight on ASC characteristics under the same genetic background using ASCs derived from monozygotic (MZ) twin pairs. Methods ASCs were isolated from subcutaneous adipose tissue from five weight-discordant (WD, within-pair difference in BMI > 3 kg/m(2)) MZ twin pairs, with measured BMI and metabolic status. The ASC immunophenotype, proliferation and osteogenic and adipogenic differentiation capacity were studied. ASC immunogenicity, immunosuppression capacity and the expression of inflammation markers were investigated. ASC angiogenic potential was assessed in cocultures with endothelial cells. Results ASCs showed low immunogenicity, proliferation, and osteogenic differentiation capacity independent of weight among all donors. ASCs showed a mesenchymal stem cell-like immunophenotype; however, the expression of CD146 was significantly higher in leaner WD twins than in heavier cotwins. ASCs from heavier twins from WD pairs showed significantly greater adipogenic differentiation capacity and higher expression of TNF and lower angiogenic potential compared with their leaner cotwins. ASCs showed immunosuppressive capacity in direct cocultures; however, heavier WD twins showed stronger immunosuppressive capacity than leaner cotwins. Conclusions Our genetically matched data suggest that a higher weight of the donor may have some effect on ASC characteristics, especially on angiogenic and adipogenic potential, which should be considered when ASCs are used clinically.
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