Journal
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
Volume 47, Issue 2, Pages 163-167Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijantimicag.2015.12.005
Keywords
Pseudomonas aeruginosa; Hyperbaric oxygen treatment; Ciprofloxacin; Biofilm; Cystic fibrosis
Funding
- UC-CARE (University of Copenhagen-Center for Antimicrobial Research) grant
- Danish Research Council for Independent Research\Technology and Production Sciences
Ask authors/readers for more resources
Chronic Pseudomonas aeruginosa lung infection is the most severe complication in cystic fibrosis patients. It is characterised by antibiotic-tolerant biofilms in the endobronchial mucus with zones of oxygen (O-2) depletion mainly due to polymorphonuclear leucocyte activity. Whilst the exact mechanisms affecting antibiotic effectiveness on biofilms remain unclear, accumulating evidence suggests that the efficacy of several bactericidal antibiotics such as ciprofloxacin is enhanced by stimulation of the aerobic respiration of pathogens, and that lack of O-2 increases their tolerance. Reoxygenation of O-2-depleted biofilms may thus improve susceptibility to ciprofloxacin possibly by restoring aerobic respiration. We tested such a strategy using reoxygenation of O-2-depleted P. aeruginosa strain PAO1 agarose-embedded biofilms by hyperbaric oxygen treatment (HBOT) (100% O-2, 2.8 bar), enhancing the diffusive supply for aerobic respiration during ciprofloxacin treatment. This proof-of-principle study demonstrates that biofilm reoxygenation by HBOT can significantly enhance the bactericidal activity of ciprofloxacin on P. aeruginosa. Combining ciprofloxacin treatment with HBOT thus clearly has potential to improve the treatment of P. aeruginosa biofilm infections. (C) 2015 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available