4.7 Article

Heat inactivation of clinical COVID-19 samples on an industrial scale for low risk and efficient high-throughput qRT-PCR diagnostic testing

Related references

Note: Only part of the references are listed.
Letter Infectious Diseases

Thermal treatment of nasopharyngeal samples before cobas SARS-CoV-2 testing

Todd M. Pryce et al.

CLINICAL MICROBIOLOGY AND INFECTION (2021)

Review Biology

Use and Misuse of Cq in qPCR Data Analysis and Reporting

Adrian Ruiz-Villalba et al.

Summary: In the analysis of qPCR data, the C-q value indicates the position of the amplification curve relative to the cycle axis, but it can vary between runs and machines and is highly influenced by PCR efficiency. To avoid misinterpretations, efficiency-corrected starting concentrations should be calculated for reporting gene expression ratios and fold differences.

LIFE-BASEL (2021)

Article Biotechnology & Applied Microbiology

Assessment of inactivation procedures for SARS-CoV-2

Heidi Auerswald et al.

Summary: Proper handling of samples infected with SARS-CoV-2 is crucial, especially in resource-limited settings where chemical and physical inactivation techniques can effectively reduce the risk of handling live virus.

JOURNAL OF GENERAL VIROLOGY (2021)

Article Multidisciplinary Sciences

Establishment and lineage dynamics of the SARS-CoV-2 epidemic in the UK

Louis du Plessis et al.

Summary: The study analyzed the genetic lineage structure of the early UK COVID-19 epidemic through genomic sampling, identifying that virus lineages introduced before the national lockdown tended to be larger and more dispersed. After the lockdown, lineage importation and regional diversity decreased, while lineage elimination depended on lineage size. The research emphasizes the impact of genetic perspective on transmission dynamics for COVID-19 epidemiology and control.

SCIENCE (2021)

Article Multidisciplinary Sciences

Test sensitivity is secondary to frequency and turnaround time for COVID-19 screening

Daniel B. Larremore et al.

Summary: The COVID-19 pandemic has led to a public health crisis due to the spread of SARS-CoV-2 through presymptomatic, symptomatic, and asymptomatic individuals. Effective population screening for the virus is essential for reopening societies and controlling its spread, with testing frequency and reporting speed playing crucial roles in its success. Test sensitivity is only marginally important compared to these factors.

SCIENCE ADVANCES (2021)

Editorial Material Medicine, General & Internal

Clarifying the evidence on SARS-CoV-2 antigen rapid tests in public health responses to COVID-19

Michael J. Mina et al.

LANCET (2021)

Article Public, Environmental & Occupational Health

Assessing critical gaps in COVID-19 testing capacity: the case of delayed results in Ecuador

Irene Torres et al.

Summary: This study found that a centralized RT-PCR testing system in Ecuador contributed to critical delays in processing, potentially masking a higher case burden than reported. Although some provinces had authorized COVID-19 processing laboratories, there is a need to estimate and augment laboratory capacities for improved decision-making and policies. Support is needed for necessary human and physical resources, as well as transportation of samples and supplies, and information management, at all phases of diagnostic testing.

BMC PUBLIC HEALTH (2021)

Review Medicine, General & Internal

The incubation period during the pandemic of COVID-19: a systematic review and meta-analysis

Wafa Dhouib et al.

Summary: Our study aimed to determine the incubation period of COVID-19 through a systematic review and meta-analysis. We analyzed 42 studies conducted mainly in China and found that the mean and median incubation period were 8 days and 12 days respectively. Parametric models showed that the 95th percentiles ranged from 10.3 to 16 days, with a 99th percentile as long as 20.4 days.

SYSTEMATIC REVIEWS (2021)

Article Biotechnology & Applied Microbiology

Heat-Treated Virus Inactivation Rate Depends Strongly on Treatment Procedure: Illustration with SARS-CoV-2

Amandine Gamble et al.

Summary: Decontamination is necessary for safe reuse of contaminated equipment and handling of biological samples. Heat treatment is a common method, but the process and container type used can greatly impact effectiveness. In reporting experimental studies and developing decontamination guidelines, specifying heat-treatment procedures and considering container types are crucial for result interpretation and reproducibility.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2021)

Article Multidisciplinary Sciences

Resilient SARS-CoV-2 diagnostics workflows including viral heat inactivation

Maria Jose Lista et al.

Summary: The study provides multiple workflows for SARS-CoV-2 nucleic acid detection in clinical samples by comparing different commercially available RNA extraction methods and RT-qPCR reagents. Beckman performed better with primer-probe combinations, and Luna reagent showed the highest sensitivity. Additionally, heat treatment of nasopharyngeal swabs can effectively inactivate SARS-CoV-2 without affecting qPCR sensitivity, making testing more portable in non-CL-3 facilities.

PLOS ONE (2021)

Review Public, Environmental & Occupational Health

COVID-19-A Novel Zoonotic Disease: A Review of the Disease, the Virus, and Public Health Measures

John S. Mackenzie et al.

ASIA-PACIFIC JOURNAL OF PUBLIC HEALTH (2020)

Letter Public, Environmental & Occupational Health

Inactivation of coronaviruses by heat

G. Kampf et al.

JOURNAL OF HOSPITAL INFECTION (2020)

Editorial Material Virology

Thermal inactivation and nucleic acid amplification-based testing for SARS-CoV-2

Helene Pere et al.

JOURNAL OF CLINICAL VIROLOGY (2020)

Article Immunology

Methods of Inactivation of SARS-CoV-2 for Downstream Biological Assays

Edward Patterson et al.

JOURNAL OF INFECTIOUS DISEASES (2020)

Letter Microbiology

How to establish an academic SARS-CoV-2 testing laboratory

Alex Richter et al.

NATURE MICROBIOLOGY (2020)

Article Multidisciplinary Sciences

SARS-CoV-2 viral load is associated with increased disease severity and mortality

Jesse Fajnzylber et al.

NATURE COMMUNICATIONS (2020)

Article Biochemical Research Methods

Evaluation of the inactivation of Venezuelan equine encephalitis virus by several common methods

Edward I. Patterson et al.

JOURNAL OF VIROLOGICAL METHODS (2018)

Article Infectious Diseases

Heat inactivation of the Middle East respiratory syndrome coronavirus

India Leclercq et al.

INFLUENZA AND OTHER RESPIRATORY VIRUSES (2014)

Article Engineering, Environmental

Virus Inactivation Mechanisms: Impact of Disinfectants on Virus Function and Structural Integrity

Krista Rule Wigginton et al.

ENVIRONMENTAL SCIENCE & TECHNOLOGY (2012)

Article Public, Environmental & Occupational Health

Inactivation of surrogate coronaviruses on hard surfaces by health care germicides

Rachel L. Hulkower et al.

AMERICAN JOURNAL OF INFECTION CONTROL (2011)

Article Biotechnology & Applied Microbiology

Effects of Air Temperature and Relative Humidity on Coronavirus Survival on Surfaces

Lisa M. Casanova et al.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2010)

Article Public, Environmental & Occupational Health

The antiviral action of common household disinfectants and antiseptics against murine hepatitis virus, a potential surrogate for SARS coronavirus

Christine Dellanno et al.

AMERICAN JOURNAL OF INFECTION CONTROL (2009)

Article Immunology

Stability and inactivation of SARS coronavirus

HF Rabenau et al.

MEDICAL MICROBIOLOGY AND IMMUNOLOGY (2005)