Journal
SCIENTIFIC REPORTS
Volume 11, Issue 1, Pages -Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41598-021-02632-1
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Funding
- Brain Korea 21 PLUS Project for Medical Science, Yonsei University
- Research Grant from Gangnam Severance Hospital, Yonsei University College of Medicine (2020)
- Yonsei University College of Medicine [6-2016-0148, 6-2020-0109]
- Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) - Ministry of Health & Welfare, Republic of Korea [HI17C1260]
- National Research Foundation of Korea [NRF-2017R1D1A1B03029560, NRF-2020R1F1A1072307]
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [NRF-2020R1A6A3A01097969]
- Research Institute of Seoul Medical Center [17-C20]
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The team proposed an optimized MAP-based method for paper-thin samples and explored its application in tissue clearing and three-dimensional imaging. Through rapid clearing and imaging, this method can be used to study the vasculature and neuronal networks of various tissues in healthy and diseased environments.
Three-dimensional visualization of cellular and subcellular-structures in histological-tissues is essential for understanding the complexities of biological-phenomena, especially with regards structural and spatial relationships and pathologlical-diagnosis. Recent advancements in tissue-clearing technology, such as Magnified Analysis of Proteome (MAP), have significantly improved our ability to study biological-structures in three-dimensional space; however, their wide applicability to a variety of tissues is limited by long incubation-times and a need for advanced imaging-systems that are not readily available in most-laboratories. Here, we present optimized MAP-based method for paper-thin samples, Paper-MAP, which allow for rapid clearing and subsequent imaging of three-dimensional sections derived from various tissues using conventional confocal-microscopy. Paper-MAP successfully clear tissues within 1-day, compared to the original-MAP, without significant differences in achieved optical-transparency. As a proof-of-concept, we investigated the vasculature and neuronal-networks of a variety of human and rodent tissues processed via Paper-MAP, in both healthy and diseased contexts, including Alzheimer's disease and glioma.
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