Journal
SCIENTIFIC REPORTS
Volume 11, Issue 1, Pages -Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41598-021-97532-9
Keywords
-
Categories
Funding
- Australian National Health and Medical Research Council (NHMRC) [GNT1173210, GNT1141441]
- BME-Nanotechnology and Materials Science FIKP grant of EMMI (BME FIKP-NAT), Hungary
- NRDI Fund (TKP2020 IES), Hungary
Ask authors/readers for more resources
The RMOD method is effective in rapidly and quantitatively diagnosing malaria, particularly in detecting Plasmodium vivax infections. Trials conducted in different transmission regions have shown its high detection potential, even at low parasite densities. Furthermore, by correlating the magnitude of the magneto-optical signal with parasite density, it is possible to estimate the relative hemozoin production rates of different stages in Plasmodium vivax infections.
The rotating-crystal magneto-optical detection (RMOD) method has been developed for the rapid and quantitative diagnosis of malaria and tested systematically on various malaria infection models. Very recently, an extended field trial in a high-transmission region of Papua New Guinea demonstrated its great potential for detecting malaria infections, in particular Plasmodium vivax. In the present small-scale field test, carried out in a low-transmission area of Thailand, RMOD confirmed malaria in all samples found to be infected with Plasmodium vivax by microscopy, our reference method. Moreover, the magneto-optical signal for this sample set was typically 1-3 orders of magnitude higher than the cut-off value of RMOD determined on uninfected samples. Based on the serial dilution of the original patient samples, we expect that the method can detect Plasmodium vivax malaria in blood samples with parasite densities as low as similar to 5-10 parasites per microliter, a limit around the pyrogenic threshold of the infection. In addition, by investigating the correlation between the magnitude of the magneto-optical signal, the parasite density and the erythrocytic stage distribution, we estimate the relative hemozoin production rates of the ring and the trophozoite stages of in vivo Plasmodium vivax infections.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available